RNA isolation / purification Cells immortalized

Get tips on using pSpCas9(BB)-2A-GFP (PX458) to perform CRISPR Human - Deletion RNase L

Get tips on using DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit to perform ROS assay cell type - H9c2 rat cardiomyocytes

Get tips on using RNeasy Mini Kit to perform

Get tips on using RNeasy Mini Kit to perform

Get tips on using DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit to perform ROS assay cell type - rat kidney and pancreas tissue

Get tips on using RNeasy Plus Mini Kit to perform

Get tips on using RNeasy Plus Mini Kit to perform

Get tips on using RNeasy Plus Mini Kit to perform

Get tips on using RNeasy Plus Mini Kit to perform AAA for reviews

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.