Protein Expression Eukaryotic cells CHO

Get tips on using DeadEnd™ Fluorometric TUNEL System to perform TUNEL assay cell type - SKOV3, Caov3 human ovarian cancer

Get tips on using ApoBrdU Red DNA Fragmentation Kit to perform TUNEL assay cell type - SKOV3, Caov3 human ovarian cancer

Get tips on using Oris™ Cell Migration Assay - Fibronectin Coated to perform Wound healing assay cell type - human Caco-2

Get tips on using BBL™ Brain Heart Infusion Broth, BD to perform Bacterial cell culture media Clostridum botulinum

Get tips on using Difco™ Brain Heart Infusion Agar, BD to perform Bacterial cell culture media Clostridium difficile

Get tips on using FITC Annexin V Apoptosis Detection Kit I to perform Apoptosis assay cell type - CaOV-3

Get tips on using PE Annexin V Apoptosis Detection Kit I to perform Apoptosis assay cell type - CaOV-3

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.