Get tips on using Cell Counting Kit-8 to perform Live / Dead assay mammalian cells - INS-1 832/12
TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.
Get tips on using Cell Counting Kit-8 to perform RNA quantification Coloremetric
Get tips on using Mesenchymal Stem Cell Chondrogenic Differentiation Medium to perform Stem cell Differentiation media hBMSCs differentiation into chondrogenic cells
Get tips on using Mesenchymal Stem Cell Chondrogenic Differentiation Medium to perform Stem cell Differentiation media hUMSCs differentiation into chondrogenic cells
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