Bacterial cell culture media

Get tips on using Gibco™ DMEM, high glucose to perform Mammalian cell culture media HAOECv

Get tips on using Nutrient Mixture F-12 Ham to perform Mammalian cell culture media BPAEC

Get tips on using Gibco DMEM/F-12, HEPES to perform Mammalian cell culture media BEnEpC

Get tips on using Gibco™ DMEM, high glucose to perform Mammalian cell culture media BCAEC

Get tips on using Gibco™ DMEM, high glucose to perform Mammalian cell culture media HaCaT

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. Multiplexing such a reaction amplifies the design challenges where one target requires 3 primers, which should be exclusively bound nowhere in the template DNA or to each other. Similarly, two targets require 6, three require 9, and so on. Each amplicon needs to be either a different size (for gels) or labeled with a different fluorescent tag that is spectrally distinct from the others in the reaction. Further complicating this, different targets in the reaction can compete with each other for resources and causes more challenges in the detection of amplicons. However, with proper primer designing, their validation, optimize quality and concentration of the enzyme and buffers certainly lead to a successful multiplex PCR reaction.

Get tips on using Advanced RPMI 1640 Medium to perform 3D Cell Culture Media hiPSC-derived kidney organoids

Get tips on using Gibco™ RPMI 1640 Medium to perform 3D Cell Culture Media Mouse thymic organoids

Get tips on using Gibco™Neurobasal™ Medium to perform 3D Cell Culture Media Mouse embryonic neurospheres

Get tips on using DMEM–Dulbecco's Modified Eagle Medium to perform Mammalian cell culture media MDA-MB-468