DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.
Get tips on using Stealth siRNA_SPI1 to perform siRNA / miRNA gene silencing Human - LAD2 PU.1/SPI1
Get tips on using siRNA Wee1 to perform siRNA / miRNA gene silencing Human - SKOV-3 Wee-1
Get tips on using siRNA RBM3 to perform siRNA / miRNA gene silencing Human - MIA PaCa-2 RBM3
Get tips on using siRNA RIP3 to perform siRNA / miRNA gene silencing Human - MIA PaCa-2 RIP3
Get tips on using siRNA Wee1 to perform siRNA / miRNA gene silencing Human - MDA-MB-231 Wee1
Get tips on using siRNA FOXM1 to perform siRNA / miRNA gene silencing Human - MDA-MB-231 FOXM1
Get tips on using Stealth Select_PTBP1 siRNA to perform siRNA / miRNA gene silencing Human - HCT-116 PTBP1
Get tips on using Hs_PTEN_6 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - HCT-116 PTEN1
Get tips on using Hs_KEAP1_5 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - BEAS-2B KEAP1
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment