Protein Expression Prokaryotic cells M. smegmatis

Get tips on using mTOR Inhibitor XI, Torin1 - Calbiochem to perform Autophagy assay cell type - GL261

Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Autophagy assay cell type - HaCaT

Get tips on using Bafilomycin A1 Ready Made Solution to perform Autophagy assay cell type - REH

Get tips on using mTOR Inhibitor XI, Torin1 - Calbiochem to perform Autophagy assay cell type - GL15

Get tips on using mTOR Inhibitor XI, Torin1 - Calbiochem to perform Autophagy assay cell type - U87MG

Get tips on using Anti-SQSTM1 / p62 antibody to perform Autophagy assay cell type - MDA-MB-231

Get tips on using SQSTM1/p62 (D5E2) Rabbit mAb to perform Autophagy assay cell type - A2780

Get tips on using SQSTM1/p62 (D1Q5S) Rabbit mAb to perform Autophagy assay cell type - A549

Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Autophagy assay cell type - HepG2

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.