siRNA / miRNA gene silencing Mouse B16 Melanoma cells

Get tips on using ANT2 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - 3T3-L1 ANT2

Get tips on using Rn_Tlr3_1 FlexiTube siRNA to perform siRNA / miRNA gene silencing Mouse - Neuro 2a TLR3

Get tips on using Atf4 siRNA(m) to perform siRNA / miRNA gene silencing Mouse - 3T3-L1 ATF4

Get tips on using Stealth siRNA(m) Stac3 to perform siRNA / miRNA gene silencing Mouse - C2C12 Stac3

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

Get tips on using Stealth siRNA(m)_Atg5 to perform siRNA / miRNA gene silencing Mouse - MLO‐Y4 Atg5

Get tips on using HIF-1α siRNA (m) to perform siRNA / miRNA gene silencing Mouse - AtT20 Hif-1alpha

Get tips on using MEK-3 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - BMDMs MEK-3

Get tips on using PU.1 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - RAW264.7 PU.1

Get tips on using Silencer® siRNA(m) Nostrin to perform siRNA / miRNA gene silencing Mouse - MS1 Nostrin