siRNA / miRNA gene silencing Human rh-36

The process of RNA extraction from bacteria, in general, involves an RNA-protective, effective lysis of bacterial cell wall (which may pose difficulties). EDTA promotes loss of outer membrane to provide lysozyme with access to peptidoglycan. Another common method for cell wall lysis is mechanical disruption using a homogenizer (applied for gram-positive bacteria and some strains of gram-negative bacteria). Following lysis, it is necessary to disrupt protein-nucleic acid interactions, which can be achieved by adding sodium dodecyl sulfate (SDS). Next step involves using phenol-chloroform-isoamyl alcohol extraction, where RNA can be obtained from the bottom organic phase, the top phase consists of DNA and the interphase contains proteins. Isoamyl alcohol is an inert and optional addition to this mixture and is added as an anti-foaming reagent to reduce the interphase. Following RNA extraction, the samples should be checked for its quality by gel electrophoresis (23S and 16S rRNAs and 5s rRNA and tRNA bands) or UV spectrophotometric or fluorescence methods.

Get tips on using ON-TARGETplus Human PAK1 (5058) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - Caco-2 PAK1

Get tips on using ON-TARGETplus Human MCL1 (4170) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - M245 MCL-1

Get tips on using ON-TARGETplus Human FOXM1 (2305) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - MCF-7 FOXM1

Get tips on using ON-TARGETplus Human ERCC4 (2072) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - U2OS XPF/ERCC4

Get tips on using ON-TARGETplus Human MOAP1 (64112) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - A2780 MOAP-1

Get tips on using ON-TARGETplus Human RAB11FIP1 (80223) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - A431 RCP/RAB11FIP1

Get tips on using ON-TARGETplus Human ABCG2 (9429) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - BC-1 BCRP

Get tips on using ON-TARGETplus Human RRM2 (6241) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - BCBL-1 RRM2

Get tips on using ON-TARGETplus Human SLC7A11 (23657) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - BCBL-1 xCT