rna-isolation-purification-tissue-mouse-mammary-glands

Get tips on using CellTiter-Glo® Luminescent Cell Viability Assay to perform Live / Dead assay mammalian cells - MC3T3

Get tips on using jetPEI® DNA transfection, HTS application to perform DNA transfection Mammalian cells - Immortalized cell lines Huh7

Get tips on using jetPEI® DNA transfection, HTS application to perform DNA transfection Mammalian cells - Primary cells Human astrocytes

Get tips on using jetPEI® DNA transfection, HTS application to perform DNA transfection Mammalian cells - Immortalized cell lines HeLa

Get tips on using jetPEI® DNA transfection, HTS application to perform DNA transfection Mammalian cells - Immortalized cell lines HepG2

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using EBMTM-2 Endothelial Cell Growth Basal Medium-2 to perform Mammalian cell culture media HCAEC

Get tips on using EGMTM-2 Endothelial Cell Growth Medium-2 BulletKit to perform Mammalian cell culture media HUVEC

Get tips on using EGMTM-2 Endothelial Cell Growth Medium-2 BulletKit to perform Mammalian cell culture media HPAEC

Get tips on using FGMTM-3 Cardiac Fibroblast Growth Medium-3 BulletKit to perform Mammalian cell culture media HCF