rna-isolation-purification-cells-primary-mouse-dorsal-root-ganglion-neurons

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TRIzol Reagent Product

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary rat astrocytes

Products Thermo Fisher Scientific TRIzol Reagent

VENTANA anti-MSH2 (G219-1129) Mouse Monoclonal Primary Antibody Product

Get tips on using VENTANA anti-MSH2 (G219-1129) Mouse Monoclonal Primary Antibody to perform Immunohistochemistry Human - MSH2

Products Roche Lifesciences VENTANA anti-MSH2 (G219-1129) Mouse Monoclonal Primary Antibody

RNA sequencing Mouse - ESCs (Embryonic Stem Cells) Experiment

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

RNA RNA sequencing Mouse ESCs (Embryonic Stem Cells)

RNeasy Mini Kit Product

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human airway epithelial cells