Get tips on using Monoclonal Anti-ATG12 antibody produced in mouse to perform Autophagy assay cell type - CaCo-2
Get tips on using EZCell™ Cell Migration/Chemotaxis Assay Kit (24-well, 8 µm) to perform Cell migration / Invasion cell type - RPMI-8226
Get tips on using EZCell™ Cell Invasion Assay (Basement Membrane), 24-well, 8 µm to perform Cell migration / Invasion cell type - RPMI-8226
Get tips on using EZCell™ Cell Invasion Assay (Basement Membrane), 24-well, 8 µm to perform Cell migration / Invasion cell type - LP-1
Get tips on using EZCell™ Cell Migration/Chemotaxis Assay Kit (24-well, 8 µm) to perform Cell migration / Invasion cell type - LP-1
Acid phosphatase detection heavily relies on determining the concentration of tartrate-resistant acid phosphatase (TRAP) in the sample. Hence, sample preparation is very crucial and it should be done strictly as per kit manufacturer instructions to avoid any inconsistency and poor sensitivity
ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.
DNA damage assay is a standard method for determining in-vivo/in-vitro genotoxicity by measuring the breaks in the DNA chain of animal and plant cells. Initial DNA damage leads to cell cycle arrest and, at the final stages, leads to induction of senescence or cell death (apoptosis, necrosis, autophagy, or mitotic catastrophe). Detection of DNA damage from mild to moderate to severe is challenging when studying genotoxicity in the pool of cells. It is favorable to use DNA damage assay kits available for prominent identification of the extent of damage in the analysis.
DNA damage assay is a standard method for determining in-vivo/in-vitro genotoxicity by measuring the breaks in the DNA chain of animal and plant cells. Initial DNA damage leads to cell cycle arrest and, at the final stages, leads to induction of senescence or cell death (apoptosis, necrosis, autophagy, or mitotic catastrophe). Detection of DNA damage from mild to moderate to severe is challenging when studying genotoxicity in the pool of cells. It is favorable to use DNA damage assay kits available for prominent identification of the extent of damage in the analysis.
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