shRNA gene silencing Mouse Prostate cancer cell lines (DU145 and PC3) CD24

Get tips on using ApopTag® Peroxidase In Situ Apoptosis Detection Kit to perform TUNEL assay cell type - FaDu human squamous cell carcinoma

Get tips on using Annexin V-FITC to perform Apoptosis assay cell type - PC-3

Get tips on using DuoSet® ELISA Development Systems to perform ELISA (kit) IL-6, IL-1β, IL-8 and TNF-α - -NA- Human -NA-

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?

Get tips on using PCR Sequencing Kit to perform Whole Genome Amplification Human

Get tips on using JNK1 Antibody (F-3) to perform Autophagy assay cell type - PC-12

Get tips on using Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) Antibody to perform Autophagy assay cell type - HepG2

Get tips on using OxiSelect™ Intracellular ROS Assay Kit (Green Fluorescence) to perform ROS assay cell type - PC12

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

Get tips on using Anti-PI3-kinase p85-α antibody produced in rabbit to perform Autophagy assay cell type - HepG2