siRNA / miRNA gene silencing Human UCC (urothelial cancer cell lines)

Get tips on using LC3A (D50G8) XP® Rabbit mAb to perform Autophagy assay cell type - Vestibular schwannoma cells

Get tips on using Anti-LC3B antibody produced in rabbit to perform Autophagy assay cell type - N27 dopaminergic cells

Get tips on using TiterTACS™ Colorimetric Apoptosis Detection Kit to perform TUNEL assay cell type - Mouse endothelial cells

Get tips on using LC3B (D11) XP® Rabbit mAb to perform Autophagy assay cell type - Vestibular schwannoma cells

Get tips on using Dulbecco’s Modified Eagle’s Medium (DMEM) (1X),liquid to perform Mammalian cell culture media HSG cells

Get tips on using LC3A/B (D3U4C) XP® Rabbit mAb to perform Autophagy assay cell type - K562 cells

Get tips on using FITC Annexin V Apoptosis Detection Kit I to perform Apoptosis assay cell type - B-cells

Get tips on using FITC Annexin V Apoptosis Detection Kit I to perform Apoptosis assay cell type - HeLa cells

Get tips on using GeneRead Size Selection Kit (50) to perform Whole Genome Amplification NGS library purification

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.