Get tips on using MouseTRAP™ (TRAcP 5b) ELISA to perform Acid phosphatase assay cell type - murine macrophage cells
Get tips on using LC3A (D50G8) XP® Rabbit mAb to perform Autophagy assay cell type - Vestibular schwannoma cells
Get tips on using Anti-LC3B antibody produced in rabbit to perform Autophagy assay cell type - N27 dopaminergic cells
Get tips on using TiterTACS™ Colorimetric Apoptosis Detection Kit to perform TUNEL assay cell type - Mouse endothelial cells
Get tips on using LC3B (D11) XP® Rabbit mAb to perform Autophagy assay cell type - Vestibular schwannoma cells
Get tips on using Dulbecco’s Modified Eagle’s Medium (DMEM) (1X),liquid to perform Mammalian cell culture media HSG cells
Get tips on using LC3A/B (D3U4C) XP® Rabbit mAb to perform Autophagy assay cell type - K562 cells
Get tips on using FITC Annexin V Apoptosis Detection Kit I to perform Apoptosis assay cell type - B-cells
Get tips on using FITC Annexin V Apoptosis Detection Kit I to perform Apoptosis assay cell type - HeLa cells
Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA.
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