siRNA / miRNA gene silencing Rat Neuronal cells

Get tips on using RevertAid RT Reverse Transcription Kit to perform cDNA synthesis Cell lines

Get tips on using iScript™ Reverse Transcription Supermix for RT-qPCR to perform cDNA synthesis Cell lines

Get tips on using pMXs-IRES-Bsd Retroviral Expression Vector to perform CRISPR Human - Repression DDX3Y

Get tips on using pMXs-IRES-Bsd Retroviral Expression Vector to perform CRISPR Human - Repression DDX3X

Get tips on using Mouse Retinol Binding Protein 4 ELISA Kit (ab202404) to perform ELISA Mouse - RBP4

Get tips on using Human Retinol binding protein 4 ELISA Kit (RBP4) (ab108897) to perform ELISA Human - RBP4

Get tips on using Human RBP4/Retinol Binding Protein 4 PicoKine™ ELISA Kit to perform ELISA Human - RBP4

Get tips on using LC3B antibody to perform Autophagy assay cell type - RT4

Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - human RT-7

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA.