Get tips on using EZCell™ Cell Migration/Chemotaxis Assay Kit (24-well, 8 µm) to perform Cell migration / Invasion cell type - RPMI-8226
Get tips on using EZCell™ Cell Invasion Assay (Basement Membrane), 24-well, 8 µm to perform Cell migration / Invasion cell type - RPMI-8226
Get tips on using EZCell™ Cell Invasion Assay (Basement Membrane), 24-well, 8 µm to perform Cell migration / Invasion cell type - LP-1
Get tips on using EZCell™ Cell Migration/Chemotaxis Assay Kit (24-well, 8 µm) to perform Cell migration / Invasion cell type - LP-1
Get tips on using Cytochrome c Releasing Apoptosis Assay Kit to perform Apoptosis assay cell type - SKOV3
Get tips on using ToxCount™ Cell Viability Assay to perform Live / Dead assay mammalian cells - glioblastoma stem cells
An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
Get tips on using Cellular ROS/Superoxide Detection Assay Kit to perform ROS assay cell type - Raw 264.7
Get tips on using In situ apoptosis detection to perform Apoptosis assay cell type - Human endometrial stromal cells
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