siRNA / RNAi /miRNA transfection Human Cells HeLa

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Mouse - C2C12

Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Rat - Gingival tissue

Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Rat - Liver tissue

Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Mouse - 3T3-L1

Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Mouse - Neuro 2a

Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Mouse - NSC-34

Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Mouse - BV-2

Get tips on using Magnetic mRNA Isolation Kit to perform RNA isolation / purification Tissue - Rat Retina