siRNA / RNAi /miRNA transfection Human Cells HeLa

Get tips on using Magnetic mRNA Isolation Kit to perform RNA isolation / purification Tissue - Rat Cerebellum

Get tips on using Magnetic mRNA Isolation Kit to perform RNA isolation / purification Tissue - Rat Brainstem

Get tips on using Magnetic mRNA Isolation Kit to perform RNA isolation / purification Tissue - Rat Brain

Get tips on using Magnetic mRNA Isolation Kit to perform RNA isolation / purification Tissue - Mouse Hippocampus

Get tips on using KAPA Stranded mRNA-Seq Kit to perform RNA sequencing Mouse - Chondrocytes

Get tips on using Magnetic mRNA Isolation Kit to perform RNA isolation / purification Tissue - Rat Pineal gland

Get tips on using PolyATtract® mRNA Isolation Systems to perform RNA isolation / purification Yeast - Coprinus cinereus

Get tips on using Dynabeads™ mRNA Purification Kit to perform RNA isolation / purification Tissue - Rat Adrenal glands

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.