DNA Damage Assay

Get tips on using Wizard® Genomic DNA Purification Kit to perform DNA isolation / purification Tissue - placenta

Get tips on using Calcein AM Cell Viability Assay Kit to perform Live / Dead assay mammalian cells - 3T3-L1

Get tips on using Cell-APOPercentage™ Apoptosis Assay to perform Apoptosis assay cell type - RAW 264.7

Get tips on using In Vitro Angiogenesis Assay Kit to perform Angiogenesis assay human - hiPSC-2-EC

Get tips on using In Vitro Angiogenesis Assay Kit to perform Angiogenesis assay human - hiPSC-1-EC

Get tips on using In Vitro Angiogenesis Assay Kit to perform Angiogenesis assay mouse - spleen-derived EPCs

Get tips on using Guava Nexin Annexin V Assay to perform Apoptosis assay cell type - HeLa cells

Get tips on using Dual-Luciferase® Reporter Assay System to perform Reporter gene assay luciferase - primary human endometrial stromal cells

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.