rna-isolation-purification-cells-immortalized-cal-27

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Get tips on using CIRCLEGROW AGAR (POWDER) to perform Bacterial cell culture media Escherichia coli

Get tips on using RPMI-1640 Medium to perform Mammalian cell culture media CML T1

Get tips on using AmpFLSTR™ Identifiler™ PCR Amplification Kit to perform Cell line authentication Ovarian cancer cell line PA-1

Get tips on using AmpFLSTR™ Identifiler™ PCR Amplification Kit to perform Cell line authentication Ovarian cancer cell line ES-2

Get tips on using Senescence β-Galactosidase Staining Kit - Cell Signaling to perform Reporter gene assay β-galactosidase substrates - mouse cardiac fibroblasts

Get tips on using Rn_Tlr3_1 FlexiTube siRNA to perform siRNA / miRNA gene silencing Mouse - Neuro 2a TLR3

Get tips on using AmpFLSTR™ Identifiler™ Plus PCR Amplification Kit to perform Cell line authentication Breast carcinoma cell line BT474

Get tips on using AmpFLSTR™ Identifiler™ Plus PCR Amplification Kit to perform Cell line authentication Endometrial Cancer cell line KLE