shRNA gene silencing Human THP-1

Get tips on using pIVEX2.4c-SnRK2.1 to perform Protein Expression Prokaryotic cells - E. coli SnRK2 A. thaliana

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Tissue - Mouse Thymus

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Tissue - Rat Thymus

Get tips on using RNeasy Plus Universal Kits to perform RNA isolation / purification Tissue - Mouse Thymus

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Tissue - Rat Thyroid gland

Get tips on using EZQ™ Protein Quantitation Kit to perform Protein quantification Tissue - mouse thymus

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Tissue - Mouse Thymus

Get tips on using DC™ Protein Assay Kit I to perform Protein quantification Tissue - mouse thymus

Get tips on using Cultrex® Collagen IV Cell Invasion Assay to perform Cell migration / Invasion cell type - TPC1

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.