The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.
Get tips on using FastLane Cell Probe Kit (200) to perform RNA isolation / purification Cells - immortalized A549
Get tips on using Lipofectamine® RNAiMAX Transfection Reagent to perform siRNA / miRNA gene silencing Human - siRNA negative control Lipid
The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.
Get tips on using FastLane Cell Probe Kit (200) to perform RNA isolation / purification Cells - immortalized HT-29
Get tips on using FastLane Cell Probe Kit (200) to perform RNA isolation / purification Cells - immortalized BxPC-3
Get tips on using RNeasy Protect Cell Mini Kit to perform RNA isolation / purification Cells - immortalized C6/36
The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.
Get tips on using REPLI-g Advanced DNA Single Cell Kit (96) to perform Whole Genome Amplification Mouse
Get tips on using QIAseq FX Single Cell DNA Library Kit (96) to perform Whole Genome Amplification Parasites
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment