ELISA (kit) IL-6, IL-1β, IL-8 and TNF-α

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Get tips on using Cell Death Detection ELISA to perform Apoptosis assay cell type - Human endometrial stromal cells

Products Sigma-Aldrich Cell Death Detection ELISA

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Proteins ELISA Human Chitinase-3-Like Protein-1 (CHI3L1) or YKL-40

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Proteins ELISA Mouse Chitinase-3-Like Protein-1 (CHI3L1) or YKL-40

Get tips on using Cell Proliferation ELISA, BrdU to perform Cell cytotoxicity / Proliferation assay cell type - MDA-MB-231 breast adenocarcenoma

Products Sigma-Aldrich Cell Proliferation ELISA, BrdU

Get tips on using Cell Cycle and Apoptosis Analysis Kit to perform Cell cycle assay human - U87

Products Beyotime Cell Cycle and Apoptosis Analysis Kit

Get tips on using Cell Cycle and Apoptosis Analysis Kit to perform Cell cycle assay human - FaDu

Products Beyotime Cell Cycle and Apoptosis Analysis Kit

Get tips on using Cell Cycle and Apoptosis Analysis Kit to perform Cell cycle assay human - HaCaT

Products Beyotime Cell Cycle and Apoptosis Analysis Kit

Get tips on using Cell Cycle and Apoptosis Analysis Kit to perform Cell cycle assay human - SKOV3

Products Beyotime Cell Cycle and Apoptosis Analysis Kit

Get tips on using Cell Death Detection ELISA to perform TUNEL assay cell type - Rat pulmonary arterial smooth muscle cells

Products Sigma-Aldrich Cell Death Detection ELISA

Get tips on using Cell Cycle and Apoptosis Analysis Kit to perform Cell cycle assay human - HT-29

Products Beyotime Cell Cycle and Apoptosis Analysis Kit

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