siRNA / miRNA gene silencing Human HeLa EPAS-1

Get tips on using ON-TARGETplus Human MID1 (4281) siRNA - Individual to perform siRNA / miRNA gene silencing Human - DuCaP MID1

Get tips on using ON-TARGETplus Human ITGB4 (3691) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - DU145 ITGB4

Get tips on using ON-TARGETplus Human PLAU (5328) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - DU145 PLAU

Get tips on using ON-TARGETplus Human EPCAM (4072) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - DU145 EPCAM

Get tips on using ON-TARGETplus Human IRF3 (3661) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - A549 IRF3

Get tips on using ON-TARGETplus Human GAPDH (2597) siRNA - Individual to perform siRNA / miRNA gene silencing Human - A549 GAPDH

Get tips on using ON-TARGETplus Human MET (4233) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - A431 MET

Get tips on using ON-TARGETplus Human PCSK6 (5046) siRNA - Individual to perform siRNA / miRNA gene silencing Human - A253 PACE4

Get tips on using ON-TARGETplus Human FURIN (5045) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - A253 Furin

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.