CRISPR Mouse Deletion RMA cells

Get tips on using FxCycle™ PI/RNase Staining Solution to perform Cell cycle assay human - HCT-116

Get tips on using FxCycle™ PI/RNase Staining Solution to perform Cell cycle assay human - HL-60

Get tips on using FxCycle™ PI/RNase Staining Solution to perform Cell cycle assay human - THP-1

Get tips on using IncuCyte® Caspase-3/7 Apoptosis Assay Reagent to perform Apoptosis assay cell type - Caspase 3/7

Get tips on using Cell Counting Kit-8 to perform RNA quantification Coloremetric

Get tips on using FastLane Cell SYBR Green Kit (200) to perform PCR Quantitative real-time PCR - Viral

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.