Immunohistochemistry Collagen Type III Goat Mouse

Get tips on using MLH1 antibody [G168-15] to perform Immunohistochemistry Human - MLH1

Get tips on using Human CRISP-3 Antibody to perform Immunohistochemistry Human - CRISP3

Get tips on using Anti-CRISP3 antibody (ab105951) to perform Immunohistochemistry Human - CRISP3

Get tips on using Anti-SOX9 Antibody AB5535 to perform Immunohistochemistry Rat - Sox9

Get tips on using Anti-GFAP antibody (ab7260) to perform Immunohistochemistry Rat - GFAP

Get tips on using Anti-MMP3 antibody (ab53015) to perform Immunohistochemistry Rat - MMP3

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Get tips on using Mouse IL-1 beta ELISA Kit (ab100704) to perform ELISA Mouse - IL-1 beta

Get tips on using Mouse ICAM-1/CD54 Quantikine ELISA Kit to perform ELISA Mouse - ICAM-1/CD54

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.