siRNA / miRNA gene silencing Human Primary Human Aortic Endothelial Cells GLO-1

Get tips on using Lipofectamine® LTX Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines BXPC3

Get tips on using LC3B Antibody Kit for Autophagy to perform Autophagy assay cell type - Lens Epithelial Cells

Get tips on using LC3B Antibody Kit for Autophagy to perform Autophagy assay cell type - HK-2 cells

Get tips on using Anti-LC3B antibody produced in rabbit to perform Autophagy assay cell type - Paneth cells

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - Mel cells

Get tips on using LC3B (D11) XP® Rabbit mAb to perform Autophagy assay cell type - SAE cells

Get tips on using FlowCellect Autophagy LC3 antibody based kit to perform Autophagy assay cell type - K562 cells

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - K562 cells

Get tips on using Anti-LC3B antibody produced in rabbit to perform Autophagy assay cell type - K562 cells

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.