Protein Expression Eukaryotic cells P. pastoris

Get tips on using TdT In Situ Apoptosis Detection Kit - Fluorescein to perform TUNEL assay cell type - Rat fibroblast-like synoviocytes

The formation of DNA from an RNA template using reverse transcription leads to the formation of double-stranded complementary DNA or cDNA. The challenges with this process include 1. Maintaining the integrity of RNA, 2. Hairpin loops or other secondary structures formed by single-stranded RNA can also affect cDNA synthesis, and 3. DNA-RNA hybrids, which may result when the first strand of cDNA is formed. For the first challenge, using workflows that involve proper isolation and storage of RNA, and maintaining a nuclease-free environment helps obtain RNA with ideal 260/230 ratios. Using a reverse transcriptase that can tolerate high temperatures (50-55oC), overcomes obstacles imposed by secondary RNA structures. Finally, RNase H has the ability to hydrolyze RNA before the formation of a second cDNA strand. It is important to ensure that RNase H activity is optimal because higher RNase H activity leads to premature degradation of the RNA template. Many reverse transcriptases offer built-in RNase H activity.

Get tips on using IMAGEN™ Parainfluenza Virus Group Kit using Direct Immunofluorescence Assay to perform Cell Culture Contamination Detection Kit Virus

Get tips on using PrepSEQ™ 1-2-3 Mycoplasma Nucleic Acid Extraction Kit to perform Cell Culture Contamination Detection Kit Mycoplasma

Get tips on using Brain heart infusion bhi agar 20 plates BD Difco BBL to perform Bacterial cell culture media Bordetella bronchiseptica

Get tips on using ShRNA CD24 Lentiviral Transduction Particles (CD24-V2LHS_71909)) to perform shRNA gene silencing Mouse - Prostate cancer cell lines (DU145 and PC3) CD24 lentiviral particles

Get tips on using ShRNA CD24 Lentiviral Transduction Particles (CD24-V2LHS_71908) to perform shRNA gene silencing Mouse - Prostate cancer cell lines (DU145 and PC3) CD24 lentiviral particles

Get tips on using Gibco™ DMEM/F-12, GlutaMAX™ supplement to perform 3D Cell Culture Media Mouse endometrium organoids

Get tips on using Gibco™ DMEM/F-12, GlutaMAX™ supplement to perform 3D Cell Culture Media Murine colonic organoids

Get tips on using Gibco™ DMEM, high glucose, GlutaMAX™ Supplement to perform 3D Cell Culture Media Murine colonic organoids