siRNA / miRNA gene silencing Human Primary Endometrial Stromal Cells hsa-miR-542-3p

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Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Tissue - Mouse Heart

Products Qiagen RNeasy Plus Mini Kit

Get tips on using Image-IT™ LIVE Green Reactive Oxygen Species Detection Kit, for microscopy to perform ROS assay cell type - H9c2 rat cardiomyocytes

Products Thermo Fisher Scientific Image-IT™ LIVE Green Reactive Oxygen Species Detection Kit, for microscopy

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Tissue - Mouse Heart

Products Thermo Fisher Scientific PureLink™ RNA Mini Kit

I am currently using a recombinant protein which shows metal-dependent DNase activity. Is it possible to pinpoint the source of the DNase activity after protein purification? More specifically, can I ensure that the DNase activity is not because of nuclease contamination from the E.coli that might have persisted and passed with the protein of interest during purification?

Discussions Is a bacterial nuclease contamination possible during protein purification?

Get tips on using AmpFLSTR™ Identifiler™ Plus PCR Amplification Kit to perform Cell line authentication Glioblastoma cell line HS683

Products Thermo Fisher Scientific AmpFLSTR™ Identifiler™ Plus PCR Amplification Kit

Protein ladders are a set of standards known as molecular weight proteins that are utilized to identify the approximate size of a protein molecule run on a PAGE gel electrophoresis. The challenges in running the ladders are the choice of appropriate protein standard as it is used as visual evidence of protein migration, transfer efficiency, and positive control. Suitable protein markers can be selected on the basis of required properties and applications, i.e., fluorescent ladder, IEF, 2D SDS-PAGE ladder, natural ladder with an isoelectric point, and optimized ladders for Western Blot chemiluminescence detection. The key factors for running a distinct protein ladder are buffer conditions, charge/voltage at migration time, and the gel's concentration.

Proteins Protein Ladder Prestained

Protein ladders are a set of standards known as molecular weight proteins that are utilized to identify the approximate size of a protein molecule run on a PAGE gel electrophoresis. The challenges in running the ladders are the choice of appropriate protein standard as it is used as visual evidence of protein migration, transfer efficiency, and positive control. Suitable protein markers can be selected on the basis of required properties and applications, i.e., fluorescent ladder, IEF, 2D SDS-PAGE ladder, natural ladder with an isoelectric point, and optimized ladders for Western Blot chemiluminescence detection. The key factors for running a distinct protein ladder are buffer conditions, charge/voltage at migration time, and the gel's concentration.

Proteins Protein Ladder Immunofluorescence

Protein ladders are a set of standards known as molecular weight proteins that are utilized to identify the approximate size of a protein molecule run on a PAGE gel electrophoresis. The challenges in running the ladders are the choice of appropriate protein standard as it is used as visual evidence of protein migration, transfer efficiency, and positive control. Suitable protein markers can be selected on the basis of required properties and applications, i.e., fluorescent ladder, IEF, 2D SDS-PAGE ladder, natural ladder with an isoelectric point, and optimized ladders for Western Blot chemiluminescence detection. The key factors for running a distinct protein ladder are buffer conditions, charge/voltage at migration time, and the gel's concentration.

Proteins Protein Ladder Unstained

Get tips on using HSP70 ELISA Kit (ab133060) to perform ELISA Mouse - HSP70

Products Abcam HSP70 ELISA Kit (ab133060)

Get tips on using HSP70 ELISA Kit (ab133060) to perform ELISA Rat - HSP70

Products Abcam HSP70 ELISA Kit (ab133060)

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