Site Directed Mutagenesis (SDM) Human Point mutation HeLa

Get tips on using ON-TARGETplus Human BSG / emmprin (682) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - BCP-1 Emmprin / BSG

Get tips on using Human PLZF Antibody to perform Immunohistochemistry Mouse - PLZF

Get tips on using Human SOX9 Antibody to perform Immunohistochemistry Mouse - SOX9

Get tips on using Human TAK1 Antibody to perform Western blotting Tak1

Flow cytometry is an immunophenotyping technique whereby sing cell suspensions are stained for either cell surface markers or intracellular proteins by fluorescently-labelled antibodies and analyzed with a flow cytometer, where fluorescently-labelled molecules are excited by the laser to emit light at varying wavelengths, which is then detected by the instrument. There are several key criteria which are required to be kept in mind while designing a flow experiment- 1. Antibody titration (optimal dilution of antibodies should be calculated in order to avoid over- or under- saturated signals for proper detection of surface and intracellular markers), 2. Precision (3 or more replicates of the sample should be used per experiment), 3. Specificity (proper isotype controls should be included in the experiment), 4. Day-to-day variability (experiments should be repeated 3 or more times to ensure consistency and avoid variability due to flow cytometer settings), 5. Antibody interaction (Fluorescence minus one or FMO should be used, which is the comparison of signals from panel minus one antibody vs. the full panel), and 6. Antibody stability (fluorescently-labelled antibodies should be stored at 4C).

Get tips on using Human VEGF ELISA Kit (ab100662) to perform ELISA Human - VEGF

Get tips on using Human VEGF 165b DuoSet ELISA to perform ELISA Human - VEGF

Get tips on using Human RBP4 Quantikine ELISA Kit to perform ELISA Human - RBP4

Get tips on using Human Prolactin/PRL ELISA Kit to perform ELISA Human - PRL

Get tips on using Human Osteopontin ELISA Kit (ab100618) to perform ELISA Human - OPN