Site Directed Mutagenesis (SDM) Human Point mutation PC-3

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using siGENOME Human PDCD1LG2 (80380) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - M244 PD-L2

Get tips on using siGENOME Human PPARD (5467) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - DU145 PPAR-delta

Get tips on using siGENOME Human ATG12 (9140) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - BT-20 Atg12

Get tips on using siGENOME Human RAB5A (5868) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - BT-20 Rab5a

Wound healing assay can be challenging due to inconsistencies and variations while making a wound on the confluent cell monolayer, consequently leads to wounds of varying sizes and widths. Moreover, this assay causes damage to the cells that are at the edge of the wound, which can prevent cell migration into the wound site and healing. The best solution is to use the standard wound healing assay kits using either combs or inserts to make a defined wound field or gap and prevent the well-to-well variation in these assays.

Get tips on using siGENOME Human ATG7 (10533) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - LN-18 ATG-7

Get tips on using siGENOME Human MAPKAPK2 (9261) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - LN-18 MK2/MAPKAPK2

Get tips on using siGENOME Human RIPK3 (11035) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - LN-18 RIP3/RIPK3

Get tips on using siGENOME Human AP1G1 (164) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - HeLa γ1-adaptin/AP1G1