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Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Tissue - Human Subcutaneous

Get tips on using PicoPure™ RNA Isolation Kit to perform RNA isolation / purification Tissue - Human Skin

Get tips on using PicoPure™ RNA Isolation Kit to perform RNA isolation / purification Tissue - Human Prostate

Get tips on using PicoPure™ RNA Isolation Kit to perform RNA isolation / purification Tissue - Human Pancreas

Get tips on using PicoPure™ RNA Isolation Kit to perform RNA isolation / purification Tissue - Human Nose

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Tissue - Human Iris

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Tissue - Human Breast

Get tips on using Qproteome Bacterial Protein Prep Kit to perform Protein isolation Bacteria - Salmonella paratyphi A

Get tips on using CytoBuster™ Protein Extraction Reagent to perform Protein isolation Mammalian cells - CHO-K1

The most widely used method for protein quantification is by spectrophotometry. The concentration of the protein in the samples is measured at an absorbance of 280 nm. The absorbance of the sample protein is then plotted against a standard curve. This method allows for total protein quantification in a sample (cell and tissue extracts). Before analysing the concentration of protein in the sample, it is important to choose the right test method.  For high protein concentration samples (above 5 - 160 mg/ml) the best method is to use the Biuret test. For low concentrations samples (between 1 - 2000µg/ml) the best methods are Lowry assay, BCA assay, Bradford assay and coomassie blue (for exact sensitivity of the test kits you use, refer to manufacturer's protocol). If the samples contain detergents like Triton X-100 then BCA assay is the best choice. For samples that have proteins larger than 3 KDa in size Bradford assay is the best choice. Each method has advantages and disadvantages, plan your analysis considering your sample characteristics.