siRNA / miRNA gene silencing Human HeLa

Get tips on using siRNA FOXM1 to perform siRNA / miRNA gene silencing Human - BT-20 FOXM1

Get tips on using ON-TARGETplus Human BSG / emmprin (682) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - BCP-1 Emmprin / BSG

Get tips on using ON-TARGETplus Human PPRC1 siRNA to perform siRNA / miRNA gene silencing Human - MCF-7 PRC (PGC-1α–related coactivator)/PPRC1

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

Get tips on using Silencer select_FGA siRNA to perform siRNA / miRNA gene silencing Human - HepG2 FGA

Get tips on using Silencer select_FGB siRNA to perform siRNA / miRNA gene silencing Human - HepG2 FGB

Get tips on using Silencer select_FGG siRNA to perform siRNA / miRNA gene silencing Human - HepG2 FGG

Get tips on using siGENOME PCNT siRNA to perform siRNA / miRNA gene silencing Human - HEK293 PCNT

Get tips on using siGENOME TRIM43 siRNA to perform siRNA / miRNA gene silencing Human - HEK293 TRIM43