siRNA / miRNA gene silencing Rat MTLn3 (rat mammary adenocarcinoma breast cancer cell line)

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using Leptin (rat), ELISA kit to perform ELISA Rat - Leptin

Get tips on using Rat Leptin ELISA Kit to perform ELISA Rat - Leptin

Get tips on using Rat Gastrin ELISA Kit to perform ELISA Rat - Gastrin

Get tips on using BDNF Rat ELISA Kit to perform ELISA Rat - BDNF

Get tips on using Rat Activin-A ELISA to perform ELISA Rat - Activin

Get tips on using Rat Adiponectin ELISA Kit to perform ELISA Rat - Adiponectin

Get tips on using Tf (Rat) ELISA Kit to perform ELISA Rat - Transferrin (Tf)