Cell cycle assay

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - mouse NIH 3T3

Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - human RT-7

Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - human BxPC-3

Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - human epidermal keratinocytes

Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - human Caco-2

Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - human MCF-7

Get tips on using DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit to perform ROS assay cell type - human primary corneal epithelial cells

Get tips on using OxiSelect™ Intracellular ROS Assay Kit (Green Fluorescence) to perform ROS assay cell type - K562 human leukemia cells

Get tips on using OxiSelect™ Intracellular ROS Assay Kit (Green Fluorescence) to perform ROS assay cell type - MDA-MB-231