rna-isolation-purification-tissue-mouse-small-intestine

Get tips on using RNeasy Plus Universal Kits to perform RNA isolation / purification Tissue - Mouse Thymus

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - Mouse Muscle

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - Mouse Liver

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - Mouse Kidney

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - Mouse Brain

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - Mouse Bone

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - Mouse Lung

Get tips on using NucleoSpin® miRNA to perform RNA isolation / purification Tissue - Mouse Brain

Get tips on using TriPure Isolation Reagent to perform RNA isolation / purification Tissue - rat pancreas tissue

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.