siRNA / RNAi /miRNA transfection Human Cells THP-1

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Get tips on using Gibco™ DMEM/F-12, GlutaMAX™ supplement to perform Stem cell culture media h-medial pallium induction and culture

Products Thermo Fisher Scientific Gibco™ DMEM/F-12, GlutaMAX™ supplement

Get tips on using Gibco™ DMEM/F-12, GlutaMAX™ supplement to perform Stem cell culture media Choroid plexus-like tissue generation from SFEBq

Products Thermo Fisher Scientific Gibco™ DMEM/F-12, GlutaMAX™ supplement

Get tips on using pLKO5.sgRNA.EFS.GFP to perform CRISPR Mouse - Activation Neuro-2a Smn1

Products Addgene pLKO5.sgRNA.EFS.GFP

Get tips on using Gibco™ DMEM/F-12, GlutaMAX™ supplement to perform Stem cell Differentiation media iPSCs or hESCs differentiation into cerebellar neuroepithelium (NE)

Products Thermo Fisher Scientific Gibco™ DMEM/F-12, GlutaMAX™ supplement

Get tips on using RPMI-1640 with Phenol Red produced by FUJIFILM Wako Pure Chemical Corporation to perform Mammalian cell culture media Ku812

Products Alpha laboratories RPMI-1640 with Phenol Red produced by FUJIFILM Wako Pure Chemical Corporation

Get tips on using Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham to perform Stem cell Differentiation media hiPSCs or hESCs differentiation to Embryoid body (EB)

Products Sigma-Aldrich Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham

Get tips on using Corning® 1L DMEM (Dulbecco’s Modified Eagle’s Medium)/F12 50:50 Mix to perform 3D Cell Culture Media Mouse fallopian organoids

Products Corning Corning® 1L DMEM (Dulbecco’s Modified Eagle’s Medium)/F12 50:50 Mix

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA.

RNA RNA isolation / purification Tissue Mouse Adipose

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA.

RNA RNA isolation / purification Tissue Mouse Adrenal glands

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA.

RNA RNA isolation / purification Tissue Mouse Artery / aorta

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