Get tips on using ELISA Kit for C Reactive Protein (CRP) to perform ELISA Rat - C-Reactive Protein/CRP
ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.
Get tips on using Cell Proliferation Reagent WST-1 to perform Cell cytotoxicity / Proliferation assay cell type - L-02
Get tips on using Cell Proliferation Reagent WST-1 to perform Cell cytotoxicity / Proliferation assay cell type - SMMC-7721, Huh7, Hep3B, 293T
Get tips on using Guava Cell Cycle Reagent for Flow Cytometry to perform Cell cycle assay human - U20S
Get tips on using Guava Cell Cycle Reagent for Flow Cytometry to perform Cell cycle assay human - A2780
Get tips on using Guava Cell Cycle Reagent for Flow Cytometry to perform Cell cycle assay human - U87
Get tips on using Guava Cell Cycle Reagent for Flow Cytometry to perform Cell cycle assay human - Jurkat
Get tips on using Guava Cell Cycle Reagent for Flow Cytometry to perform Cell cycle assay human - K562
Get tips on using Guava Cell Cycle Reagent for Flow Cytometry to perform Cell cycle assay human - SKOV3
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