Stem cell culture media

Get tips on using HyClone™ Dulbecco's Modified Eagles Medium to perform Mammalian cell culture media MCF-7

Get tips on using HyClone™ Dulbecco's Modified Eagles Medium to perform Mammalian cell culture media PANC-1

Get tips on using MGMTM-4 Melanocyte Growth Medium-4 BulletKitTM to perform Mammalian cell culture media HEM

Get tips on using EpiLife™ Medium, with 60 µM calcium to perform Mammalian cell culture media HEK

Get tips on using Gibco™IMDM, powder to perform Stem cell Differentiation media hPSCs or iPSCs differentiation into Lung progenitor cells

Get tips on using Gibco™DMEM/F-12 to perform Stem cell Differentiation media iPSCs or hESCs differentiation into Neuronal cells

Get tips on using Gibco™ StemPro™ hESC SFM to perform Stem cell Differentiation media hiPSCs or hESCs differentiation to Embryoid body (EB)

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using Dulbecco’s Modified Eagle’s Medium (DMEM) (1X),liquid to perform Mammalian cell culture media HSG cells