siRNA / RNAi /miRNA transfection Human Cells THP-1

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Get tips on using lenti sgRNA(MS2)_zeo backbone to perform CRISPR Mouse - Activation C2C12 FST

Products Addgene lenti sgRNA(MS2)_zeo backbone

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA.

RNA RNA isolation / purification Tissue Mouse Blood / serum / plasma / buffy coat

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA

RNA RNA isolation / purification Tissue Rat Blood / Serum / Plasma / Buffy coat

Get tips on using RNAqueous™ Total RNA Isolation Kit to perform RNA isolation / purification Tissue - Mouse Uterus

Products Thermo Fisher Scientific RNAqueous™ Total RNA Isolation Kit

I would like to excise a large strand of DNA and insert a new one using CRISPR. My problem is that my strand will be a little over 1kb and I am not sure if this is going to be a limiting factor. Also, how long should the homology arms be for a region of this size?

Discussions DNA insert using CRISPR

Get tips on using RNAprotect Bacteria Reagent to perform RNA stabilization Microbial

Products Qiagen RNAprotect Bacteria Reagent

Get tips on using ExpressArt FFPE Clear RNAready to perform RNA isolation / purification Tissue - Rat Kidney

Products Amsbio ExpressArt FFPE Clear RNAready

Get tips on using illustra RNAspin Mini Kit to perform RNA isolation / purification Tissue - Mouse Jejunum

Products GE Healthcare Life Sciences illustra RNAspin Mini Kit

Get tips on using RNAGEM™ Tissue PLUS to perform RNA isolation / purification Tissue - Rat Pineal gland

Products ZyGEM RNAGEM™ Tissue PLUS

Get tips on using ExpressArt FFPE Clear RNAready to perform RNA isolation / purification Tissue - rat kidney tissue

Products Amsbio ExpressArt FFPE Clear RNAready

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