Get tips on using lenti sgRNA(MS2)_zeo backbone to perform CRISPR Mouse - Activation C2C12 FST
Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA.
Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA
Get tips on using RNAqueous™ Total RNA Isolation Kit to perform RNA isolation / purification Tissue - Mouse Uterus
I would like to excise a large strand of DNA and insert a new one using CRISPR. My problem is that my strand will be a little over 1kb and I am not sure if this is going to be a limiting factor. Also, how long should the homology arms be for a region of this size?
Get tips on using RNAprotect Bacteria Reagent to perform RNA stabilization Microbial
Get tips on using ExpressArt FFPE Clear RNAready to perform RNA isolation / purification Tissue - Rat Kidney
Get tips on using illustra RNAspin Mini Kit to perform RNA isolation / purification Tissue - Mouse Jejunum
Get tips on using RNAGEM™ Tissue PLUS to perform RNA isolation / purification Tissue - Rat Pineal gland
Get tips on using ExpressArt FFPE Clear RNAready to perform RNA isolation / purification Tissue - rat kidney tissue
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