ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.
ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.
ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.
Get tips on using Rat Endothelial Cell Growth Medium to perform Mammalian cell culture media RAOEC
Get tips on using Radius™ 96-Well Cell Migration Assay to perform Cell migration / Invasion cell type - FaDu
Get tips on using Radius™ 24-Well Cell Migration Assay to perform Cell migration / Invasion cell type - SH-SY5Y
Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Cell cytotoxicity / Proliferation assay cell type - K562
Get tips on using Atg12 (D88H11) Rabbit mAb to perform Autophagy assay cell type - MT-2 (human T cell leukaemia)
Get tips on using Atg7 (D12B11) Rabbit mAb to perform Autophagy assay cell type - MT-2 (human T cell leukaemia)
Get tips on using Atg5 (D5F5U) Rabbit mAb to perform Autophagy assay cell type - MT-2 (human T cell leukaemia)
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment