siRNA / RNAi /miRNA transfection Human Cal 27 cells

Get tips on using Advanced DMEM to perform Mammalian cell culture media HC

Get tips on using Medium 231 to perform Mammalian cell culture media HAOSMC

Get tips on using Medium 200 to perform Mammalian cell culture media HAOECv

Get tips on using Medium 231 to perform Mammalian cell culture media BPAEC

Get tips on using RPMI 1640 to perform Mammalian cell culture media HepG2

Get tips on using Keratinocyte-SFM to perform Mammalian cell culture media HaCaT

Get tips on using Medium 231 to perform Mammalian cell culture media DAOY

Get tips on using RPMI 1640 to perform Mammalian cell culture media A2780

Get tips on using RPMI 1640 to perform Mammalian cell culture media CHO

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.