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Wound healing assay

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Cellular assays Cell cytotoxicity / Proliferation assay cell type HCT116

Get tips on using Pierce™ Coomassie (Bradford) Protein Assay Kit to perform Protein quantification Mammalian cells - MDA-MB-231

Products Thermo Fisher Scientific Pierce™ Coomassie (Bradford) Protein Assay Kit

Get tips on using 96-Well Cell Invasion Assay, Basement Membrane to perform Cell migration / Invasion cell type - RAW 264.7

Products Cell Biolabs 96-Well Cell Invasion Assay, Basement Membrane

Get tips on using Cultrex BME Cell Invasion Assay, 96 well to perform Cell migration / Invasion cell type - HT-1080

Products R&D Systems Cultrex BME Cell Invasion Assay, 96 well

Get tips on using 96-Well Cell Invasion Assay, Collagen I to perform Cell migration / Invasion cell type - MCF-10A

Products Cell Biolabs 96-Well Cell Invasion Assay, Collagen I

Get tips on using 96-Well Cell Invasion Assay, Basement Membrane to perform Cell migration / Invasion cell type - OACM5.1 C

Products Cell Biolabs 96-Well Cell Invasion Assay, Basement Membrane

Get tips on using Radius™ 24-Well Cell Migration Assay to perform Cell migration / Invasion cell type - SH-SY5Y

Products Cell Biolabs Radius™ 24-Well Cell Migration Assay

Get tips on using β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer to perform Reporter gene assay β-galactosidase substrates - human MSCs (mesenchymal stem cells)

Products Promega β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer

Get tips on using Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells to perform Live / Dead assay mammalian cells - FE002-SK2 human skin progenitor cells

Products Biotium Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Cellular assays Live / Dead assay mammalian cells mouse splenocytes

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