siRNA / miRNA gene silencing Human SUIT-2

Get tips on using Hs_IRF7_1 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - HT-29 IRF7

Get tips on using Hs_IRF3_4 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - HT-29 IRF3

Get tips on using Hs_TLR3_5 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - HT-29 TLR3

Get tips on using Nox3 siRNA (h) to perform siRNA / miRNA gene silencing Human - BEAS-2B NOX3

Get tips on using Nrf2 siRNA (m) to perform siRNA / miRNA gene silencing Human - BEAS-2B Nrf

Get tips on using siRNA p62 / SQSTM1 to perform siRNA / miRNA gene silencing Human - BEAS-2B P62

Get tips on using SignalSilence® IκBα siRNA to perform siRNA / miRNA gene silencing Human - HT-29 IκBα

Get tips on using ON-TARGETplus Human RAB11FIP1 (80223) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - MDA-MB-231 Rab Coupling Protein (RCP)

Get tips on using ON-TARGETplus Human LIN28A (79727) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - hES cell line H1 (WA01) LIN28

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.