siRNA / miRNA gene silencing Rat RGC-5

Get tips on using ERK 1 siRNA (h) to perform siRNA / miRNA gene silencing Human - NHLF ERK1

Get tips on using ENX-1 siRNA (h) to perform siRNA / miRNA gene silencing Human - HaCaT EZH2

Get tips on using Flotillin-1 siRNA (h) to perform siRNA / miRNA gene silencing Human - A2780 FLOT1

Get tips on using Dlx-2 siRNA (h) to perform siRNA / miRNA gene silencing Human - A549 DLX2

Get tips on using siGENOME Human PAK1 siRNA to perform siRNA / miRNA gene silencing Human - HeLa PAK1

Get tips on using SnoA/N siRNA (h) to perform siRNA / miRNA gene silencing Human - SW1990 SnoN

Get tips on using Estrogen Receptor alpha siRNA (m) to perform siRNA / miRNA gene silencing Mouse - mESC ERα

Get tips on using ON-TARGETplus Human RAB25 (57111) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - ES2 RAB25

RNAi or RNA interference is a common method to suppress gene expression in vitro/in vivo by utilizing the inherent microRNA machinery, without introducing a total gene knockout. miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with the desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid-mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time-consuming, but provide a more permanent expression of RNAi in the cells and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines.

Get tips on using Pre-designed and validated siRNA against gene IGFBP1 to perform siRNA / miRNA gene silencing Human - Primary Endometrial Stromal Cells IGFBP1 (Insuline-like growth factor binding protein-1)