CRISPR Rat Deletion INS-1 832/13

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

Get tips on using β-2-Microglobulin Antibody (BBM.1): sc-13565 to perform Western blotting β₂ microglobulin

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

Get tips on using Fluorescent 1 kb DNA Ladder to perform DNA Ladder 1 kb

Get tips on using 1 kb Plus DNA Ladder to perform DNA Ladder 1 kb

Get tips on using Human ICAM-1/CD54 Antibody to perform Western blotting ICAM-1

Get tips on using Mouse KIM-1 ELISA Kit to perform ELISA Mouse - KIM-1

Get tips on using Endothelin-1 Quantikine ELISA Kit to perform ELISA Mouse - Endothelin 1

Get tips on using Endothelin 1 ELISA Kit (ab133030) to perform ELISA Mouse - Endothelin 1