siRNA / miRNA gene silencing Human hES cell line H1 (WA01)

Get tips on using Hs_HDAC2_1 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - LOR-L23 HDAC2_1

Get tips on using Hs_HDAC1_6 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - LOR-L23 HDAC1_6

Get tips on using Hs_BAP1_5 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - LOR-L23 BAP1_5

Get tips on using Hs_BAP1_3 FlexiTube siRNA to perform siRNA / miRNA gene silencing Human - LOR-L23 BAP1_3

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Get tips on using siRNA Wee1 to perform siRNA / miRNA gene silencing Human - SKOV-3 Wee-1

Get tips on using siRNA RBM3 to perform siRNA / miRNA gene silencing Human - MIA PaCa-2 RBM3

Get tips on using siRNA RIP3 to perform siRNA / miRNA gene silencing Human - MIA PaCa-2 RIP3

Get tips on using siRNA Wee1 to perform siRNA / miRNA gene silencing Human - MDA-MB-231 Wee1

Get tips on using siRNA FOXM1 to perform siRNA / miRNA gene silencing Human - MDA-MB-231 FOXM1