RNA isolation / purification Cells Cancer cell lines Leukemia cancer cell lines

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. Multiplexing such a reaction amplifies the design challenges where one target requires 3 primers, which should be exclusively bound nowhere in the template DNA or to each other. Similarly, two targets require 6, three require 9, and so on. Each amplicon needs to be either a different size (for gels) or labeled with a different fluorescent tag that is spectrally distinct from the others in the reaction. Further complicating this, different targets in the reaction can compete with each other for resources and causes more challenges in the detection of amplicons. However, with proper primer designing, their validation, optimize quality and concentration of the enzyme and buffers certainly lead to a successful multiplex PCR reaction.

Get tips on using Senescence β-Galactosidase Staining Kit - Beyotime to perform Reporter gene assay β-galactosidase substrates - mouse pancreatic stellate cells

Get tips on using β-Gal Reporter Gene Assay, chemiluminescent to perform Reporter gene assay β-galactosidase substrates - mouse mesenchymal stem cells

Get tips on using Luminescent β-galactosidase Detection Kit II to perform Reporter gene assay β-galactosidase substrates - mouse mesenchymal stem cells

Get tips on using Imprint® Methylated DNA Quantification Kit to perform DNA methylation profiling Whole genome profiling - C2C12 mouse myoblast cells

Get tips on using ONE-Glo™ Luciferase Assay System to perform Reporter gene assay luciferase - BHK-21 baby hamster kidney cells

Get tips on using SuperLight™ Dual Luciferase Reporter Gene Assay Kit to perform Reporter gene assay luciferase - HepG2 and Huh7 cells

I intend to use iScript cDNA Synthesis Kit in order to synthesize cDNA for qPCR. I have confirmed that my RNA is pure however, according to my extraction protocol I have suspended the RNA in TE buffer containing 1mM EDTA. Will the presence of EDTA have an effect on cDNA synthesis?

Get tips on using MessageAmp II aRNA Amplification Kit to perform Microarray RNA amplification & Labeling - Rhesus monkey brain tissue Biotin

Get tips on using MessageAmp II aRNA Amplification Kit to perform Microarray RNA amplification & Labeling - Human brain tissue Cyanine 3