siRNA / miRNA gene silencing Human Primary Human Aortic Endothelial Cells GLO-1

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Western blotting MMP-13 Experiment

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Proteins Western blotting MMP-13

100 bp DNA ladder-TLAD-100 Product

Get tips on using 100 bp DNA ladder-TLAD-100 to perform DNA Ladder 100 bp

Products Biomall.in 100 bp DNA ladder-TLAD-100

mRNA / Ribonucleoprotein isolation / purification Ribonucleoprotein Experiment

RNA mRNA / Ribonucleoprotein isolation / purification Ribonucleoprotein

TruSeq Stranded mRNA Product

Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Mouse - C2C12