ChIP acH3 Rat Mouse

Get tips on using ON-TARGETplus Mouse Lgals3 siRNA to perform siRNA / miRNA gene silencing Mouse - BV2 LGAL3S3

Get tips on using ON-TARGETplus Mouse Samhd1 siRNA to perform siRNA / miRNA gene silencing Mouse - BMDMs SAMHD1

The process of RNA extraction from bacteria, in general, involves an RNA-protective, effective lysis of bacterial cell wall (which may pose difficulties). EDTA promotes loss of outer membrane to provide lysozyme with access to peptidoglycan. Another common method for cell wall lysis is mechanical disruption using a homogenizer (applied for gram-positive bacteria and some strains of gram-negative bacteria). Following lysis, it is necessary to disrupt protein-nucleic acid interactions, which can be achieved by adding sodium dodecyl sulfate (SDS). Next step involves using phenol-chloroform-isoamyl alcohol extraction, where RNA can be obtained from the bottom organic phase, the top phase consists of DNA and the interphase contains proteins. Isoamyl alcohol is an inert and optional addition to this mixture and is added as an anti-foaming reagent to reduce the interphase. Following RNA extraction, the samples should be checked for its quality by gel electrophoresis (23S and 16S rRNAs and 5s rRNA and tRNA bands) or UV spectrophotometric or fluorescence methods.

Get tips on using PE Hamster Anti-Mouse CD279 to perform Flow cytometry Anti-bodies Mouse - CD279/PD-1

Get tips on using APC anti-mouse CD140a Antibody to perform Flow cytometry Anti-bodies Mouse - CD140/PDGFR-α

Get tips on using Biotin anti-mouse CD106 Antibody to perform Flow cytometry Anti-bodies Mouse - CD106/Vcam-1

Get tips on using APC anti-mouse CD31 Antibody to perform Flow cytometry Anti-bodies Mouse - CD31/Pecam-1

Get tips on using Atg12 Antibody (Mouse Specific) #2011 to perform Autophagy assay cell type - MEFs (mouse embryonic fibroblasts)

Get tips on using Purified Mouse Anti-Nucleoporin p62 to perform Western blot p62/SQSTM1 - Mouse IgG2b Human -NA-

Get tips on using ON-TARGETplus Mouse Jun siRNA to perform siRNA / miRNA gene silencing Mouse - Embryonic stem cells Jun